Transcription and Translation Reagents
New England Biolabs, Inc. m7G(5')ppp(5')A RNA Cap Structure Analog – 5 umol
The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas. Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA polymerase primed with m7G(5' )ppp(5' )G.
New England Biolabs, Inc. ProtoScript II Reverse Transcriptase – 40000 units
ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.
Trilink Bio Technologies Inc CleanCap Reagent AG (3' OMe), 1 µmole
Molecular Formula C33H45N15O24P4 (free acid), Purity ≥95% by AX-HPLC, Molecular Weight 1159.60, CleanCap Reagent AG (3' OMe) is TriLink’s proprietary co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. CleanCap has shown to provide up to 98% capping efficiency1. CleanCap AG (3' OMe) includes the 5' N7-Methyl-3'-O-Methylguanosine commonly found in mRNA capped using ARCA, whereas CleanCap AG (N-7113) has a naturally occurring 5' N7-Methylguanosine structure.
HORIZON DISCOVERY BIOSCIENCES LIMITED CRISPRMOD CRISPRA DCAS9-,100UG
CRISPRmod CRISPRa dCas9-VPR mRNA, 100ugA DNA-free option for dCas9-VPR expressionPurified dCas9-VPR mRNA for co-transfection or electroporation with synthetic CRISPRa crRNA for gene activation
Cellecta Inc CRISPR DCAS9-VPH EXPRSS VECTOR
CRISPR dCas9-VPH expression vector lentivirus (1x106^ TU)
CDW Transcend MTS430S - solid state drive - 256 GB - SATA 6Gb/s
Transcend's ultra-compact M.2 SSD 430S, adopting the SATA III 6Gb/s interface and DDR3 DRAM cache, is well-suited to address the high-performance needs and strict size limitations of small form factor devices. By using only high-quality NAND flash and enhanced firmware algorithms, Transcend's M.2 SSD 430S delivers peerless reliability.
HORIZON DISCOVERY BIOSCIENCES LIMITED CRISPRI DCAS9-SALL1-SDS3 MRNA
NC2338306 CRISPRI DCAS9-SALL1-SDS3 MRNA
McKesson General Medical LINEARITY KIT RETICULOCYTE CON
Linearity Kit Reticulocyte Concentrations 7 X 2.5 mL
HORIZON DISCOVERY BIOSCIENCES LIMITED CRISPRMOD CRISPRI HUMAN SLC33A
NC2194117 CRISPRMOD CRISPRI HUMAN SLC33A
HORIZON DISCOVERY BIOSCIENCES LIMITED DCAS9-SALL1-SDS3 LNTVL PT 50UL
CRISPRmod CRISPRi Lentiviral hCMV-Blast-dCas9-SALL1-SDS3 Particles 50ul
HORIZON DISCOVERY BIOSCIENCES LIMITED EDIT-R SYNTHETIC SGRNA NON-TAR
NC2151284 EDIT-R SYNTHETIC SGRNA NON-TAR